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n-Dodecyl-B-D-maltoside (DDM) | GoldBio- ddm detergent stock solution ,GoldBio’s n-Dodecyl-B-D-maltoside is a detergent ideal for solubilizing hydrophobic membrane proteins and preserves protein activity.Digitonin (5%) - Thermo Fisher ScientificRecommendations. 5% Digitonin is a detergent solution used to prepare native protein samples for use with the NativePAGE™ Gel System. Also included in the NativePAGE™ Sample Prep Kit, this ready-to-use detergent solution improves solubility of hydrophobic and membrane proteins during sample preparation. The samples prepared with 10% DDM, 5% ...



What is the stock and working concentration of DDM (n ...

2) Could a stock solution be prepared of DDM and stored for further use? I am using the detergent for the first time and would like to know whether it would be stable when prepared and stored for ...

28-9833-26 AA DF Membrane Protein Purification Kit - final

DDM DM LDAO FOS12 C 12 E 8 CYMAL-5 OG DDM DM LDAO FOS12 C 12 E 8 CYMAL-5 OG Solubilization Mix membranes + detergent stock solution Incubate at room temperature Clarification Centrifuge at 50 000 × g Purification (IMAC) Collect supernatants and add His Mag Sephar ose Ni into each detergent solubilized membrane protein sample Analysis of target ...

Dodecyl Beta-D-Maltoside - an overview | ScienceDirect Topics

Determination of the appropriate detergent con-. centration is important, since the complete extraction of the functional enzyme depends on detergent/protein ratio. 1. Resuspend 30 ml of crude membranes in 1.5% n -dodecyl- β -D-maltoside (DDM), 350 m M NaCl, 50 m M KP i 7.3, and 0.01% PMSF at a final volume of 210 mI.

Dividend Discount Model (DDM) of Stock Valuation

Oct 14, 2017·This presentation covers the basics of Dividend Discount Model (DDM). Firstly, fundamental formula for valuing a stock using DDM is discussed. After that, 3 cases i.e DDM for zero growth, constant growth, and variable growth stocks, are discussed.

Modular detergents tailor the purification and structural ...

Jan 28, 2020·Membrane aliquots (0.5 mL) were treated with buffer B (3.5 mL) and detergent stock solution (1 mL, c = 5w%, in water). Mixtures were agitated for 16 h at 4 °C.

A rapid expression and purification condition screening ...

The FSEC was performed in running buffer H7‐DDM (25 mM HEPES pH 7.0, 200 mM NaCl, 0.03% DDM). The flow rate was 1 mL/min. For the detergent comparison data in Figure 7, all detergents were added to the FSEC running buffer at a concentration of ∼3× their respective c.m. (See Table SI for critical micelle concentrations).

Stock Solutions 101: Everything You Need to Know

X solutions. One of the most common X solutions is 10X PBS (phosphate buffered saline), a buffer solution commonly used in biological research. In preparing such solutions, you need to measure 10 times the amount of each solute to come up with a highly concentrated stock solution. Tips in Preparing Stock Solutions. Use a proven recipe.

Digitonin - an overview | ScienceDirect Topics

Digitonin, which precipitates at 4°C and also partly at room temperature, is redissolved by heating shortly before use. Stock solutions of Tricine (1 M), 6-aminocaproic acid (2 M), imidazole (1 M), and imidazole/HCl (1 M; pH 7.0) are stored at 7°C. Buffers for BN-PAGE and further stock solutions …

Modular detergents tailor the purification and structural ...

Jan 28, 2020·Membrane aliquots (0.5 mL) were treated with buffer B (3.5 mL) and detergent stock solution (1 mL, c = 5w%, in water). Mixtures were agitated for 16 h at 4 °C.

What concentration of dodecyl maltoside (DDM) detergent to ...

2) Could a stock solution be prepared of DDM and stored for further use? I am using the detergent for the first time and would like to know whether it would be stable when prepared and stored for ...

10:1 DDM/CHS Pre-Made Solution - Anatrace

50 ml. 337. Units. Add to cart. Product Description Technical Documentation Required / Related Products. Formulation components – Dodecyl Maltoside (D310) and Cholesteryl Hemisuccinate (CH210) Formulation -. o 10:1 Dodecyl Maltoside to Cholesteryl Hemisuccinate (CH210) in deionized water. o Final concentration 10% Dodecyl Maltoside (D310) to ...

Production of Recombinant Transmembrane Proteins from ...

Add 6 ml of 10% DDM stock to make a final of 1.5% in 40 ml. Mix on a rotator at moderate speed for 2 hrs at 4°C. Meanwhile take 1.5 ml of TALON resin slurry and rinse with water and SB. Move the resin into a fresh 50 ml conical tube. Add 2 ml of SB and DDM from stock to make a final concentration of 0.5% detergent.

Rapid and improved reconstitution of bacterial ...

Purified MscS was taken from a stock solution containing the protein dissolved in PBS and 10 mM DDM detergent and was incor-porated into giant artificial liposomes, which were prepared using a modified procedure [15,16]. Two hundred microliters of a 10 mg/ mL solution of azolectin in chloroform was dried in a test tube un-der a stream of N

Stock Solutions 101: Everything You Need to Know

X solutions. One of the most common X solutions is 10X PBS (phosphate buffered saline), a buffer solution commonly used in biological research. In preparing such solutions, you need to measure 10 times the amount of each solute to come up with a highly concentrated stock solution. Tips in Preparing Stock Solutions. Use a proven recipe.

Calculating Liquid Chemical Dilutions - RPC-Rabrenco

Concentration of stock solution is 100 % Flocide 375. Total amount of 1 % solution desired is 5 gallons. - 5 gallons is equal to 640 fluid ounces (fl.oz). - 1 % of the 640 fl.oz solution must be stock Flocide 375. - 1 % (0.01) x 640 = 6.4 fl.oz of the solution must be Flocide 375 and the remainder is

28-9833-26 AA DF Membrane Protein Purification Kit - final

DDM DM LDAO FOS12 C 12 E 8 CYMAL-5 OG DDM DM LDAO FOS12 C 12 E 8 CYMAL-5 OG Solubilization Mix membranes + detergent stock solution Incubate at room temperature Clarification Centrifuge at 50 000 × g Purification (IMAC) Collect supernatants and add His Mag Sephar ose Ni into each detergent solubilized membrane protein sample Analysis of target ...

Slow Phospholipid Exchange between a Detergent-Solubilized ...

Jan 24, 2017·This dilution effect only becomes somewhat significant when adding excess DDM at 3.2 mg/mL (32 μL of a 200 mg/mL stock solution resulting in a 1.6% dilution). Numbers indicate the concentrations of detergent and lipid added at each step to the cuvette, in mg/mL.

How do you choose the detergent concentration for protein ...

Jan 01, 2009·2) Could a stock solution be prepared of DDM and stored for further use? I am using the detergent for the first time and would like to know whether it …

The GFP thermal shift assay for screening ligand and lipid ...

Oct 27, 2021·DDM stock. 10% (wt/vol) in H 2 O. Can be stored at −20 °C for up to 6 months; use up in fewer than five freeze–thaw cycles. OG stock. 10% (wt/vol) in H 2 O. Can be stored at −20 °C for up ...

Monitoring protein conformational changes and dynamics ...

May 08, 2014·Detergent solution. This solution is a 10% (wt/vol) stock solution of DDM. Store it at −20 °C in 0.5-ml aliquots for 6 months. Protein solubilization solution.

Role of Detergents in Conformational Exchange of a G ...

of DDM. High detergent off-rates are more likely to facilitate conformational exchange between distinct functional states associated with the G protein-coupled receptor. ... pared by diluting the stock solution (100 nM MNG-3) using 20 mM HEPES (pH 7.5) and 100 mM NaCl. Each sample was eval-

ANIOS DDM ECO détergent, dégraissant, désinfectant alimentaire

L’ ANIOS DDM ECO est un détergent, dégraissant, désinfectant concentré à 1 ou 2 %. Il possède un très large spectre de désinfection, avec un taux de dilution est de 1 à 2%. Sa norme EN14476 le rend efficace sur les virus enveloppés comme le coronavirus avec un taux de 1,5% et …

Characterising protein/detergent complexes by triple ...

Feb 15, 2016·For DDM, unfolded detergent-free Mistic and DDM micelles co-elute . ... To this end, a detergent (here, LDAO) stock solution is used to minimise non-systematic errors. Solution C (LDAO stock solution): Weigh 57.35 mg LDAO and dissolve it in 10 mL solution …

Dodecyl-beta-D-maltoside | C24H46O11 - PubChem

Dodecyl Maltoside is an alkyl disaccharide compound and polar surfactant, with potential adenoviral transduction-enhancing activity. Upon administration as a bladder wash, dodecyl maltoside (DDM) acts as a surfactant and enhances adenoviral transduction and infection of the bladder urothelium.

Purification and characterization of the human adenosine ...

Oct 30, 2003·Water was added afterwards to give a final volume of 315 mL. Then 35 mL of detergent stock solution (10% DDM, 2% CHS) were added with stirring. The suspension was sonicated with stirring on ice, using a sonicator ultrasonic processor XL (Misonix, Farmingdale, NY, USA), level 5, pulsing 1 s on/1 s off, with 12 s pulsing on per gram of cells.

David L. Stokes, William J. Rice, Minghui Hu, Changki Kim ...

stock solution into a glass test tube (7 cm × 1 cm) or small round bottom flask (25ml) (see Notes 1 and 2). Rotate the flask rapidly to swirl the solution around to the bottom while blowing N 2 gas across the solution to evaporate the chloro-form. This procedure should produce a thin, even film of dry lipid on the bottom of the tube.